ebm2 growth media Search Results


endothelial cell basal medium 2  (PromoCell)
99
PromoCell endothelial cell basal medium 2
Endothelial Cell Basal Medium 2, supplied by PromoCell, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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endothelial cell basal medium 2 - by Bioz Stars, 2026-03
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serum free medium  (Thermo Fisher)
96
Thermo Fisher serum free medium
Serum Free Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
serum free medium - by Bioz Stars, 2026-03
96/100 stars
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endothelial growth media 2 microvascular egm2-mv  (Lonza)
90
Lonza endothelial growth media 2 microvascular egm2-mv
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Endothelial Growth Media 2 Microvascular Egm2 Mv, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endothelial growth media 2 microvascular egm2-mv/product/Lonza
Average 90 stars, based on 1 article reviews
endothelial growth media 2 microvascular egm2-mv - by Bioz Stars, 2026-03
90/100 stars
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egm2mv  (Lonza)
90
Lonza egm2mv
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Egm2mv, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/egm2mv/product/Lonza
Average 90 stars, based on 1 article reviews
egm2mv - by Bioz Stars, 2026-03
90/100 stars
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endothelial cell growth media kits ebm-2  (Lonza)
90
Lonza endothelial cell growth media kits ebm-2
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Endothelial Cell Growth Media Kits Ebm 2, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endothelial cell growth media kits ebm-2/product/Lonza
Average 90 stars, based on 1 article reviews
endothelial cell growth media kits ebm-2 - by Bioz Stars, 2026-03
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endothelial growth media-2 singlequot  (Cambrex)
90
Cambrex endothelial growth media-2 singlequot
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Endothelial Growth Media 2 Singlequot, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endothelial growth media-2 singlequot/product/Cambrex
Average 90 stars, based on 1 article reviews
endothelial growth media-2 singlequot - by Bioz Stars, 2026-03
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singlequot media bullet kit  (Cambrex)
90
Cambrex singlequot media bullet kit
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Singlequot Media Bullet Kit, supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/singlequot media bullet kit/product/Cambrex
Average 90 stars, based on 1 article reviews
singlequot media bullet kit - by Bioz Stars, 2026-03
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ebm 2 mv media  (PromoCell)
98
PromoCell ebm 2 mv media
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Ebm 2 Mv Media, supplied by PromoCell, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ebm 2 mv media/product/PromoCell
Average 98 stars, based on 1 article reviews
ebm 2 mv media - by Bioz Stars, 2026-03
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ebm 2 mv growth media  (Lonza)
99
Lonza ebm 2 mv growth media
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Ebm 2 Mv Growth Media, supplied by Lonza, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ebm 2 mv growth media/product/Lonza
Average 99 stars, based on 1 article reviews
ebm 2 mv growth media - by Bioz Stars, 2026-03
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ebm 2 growth media  (Lonza)
96
Lonza ebm 2 growth media
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Ebm 2 Growth Media, supplied by Lonza, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ebm 2 growth media/product/Lonza
Average 96 stars, based on 1 article reviews
ebm 2 growth media - by Bioz Stars, 2026-03
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endothelial growth media-2 singlequot (ebm-2, cc-3156)  (Cambrex)
90
Cambrex endothelial growth media-2 singlequot (ebm-2, cc-3156)
hESC monolayer differentiation. hESC were differentiated in <t>EGM2-MV</t> for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).
Endothelial Growth Media 2 Singlequot (Ebm 2, Cc 3156), supplied by Cambrex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/endothelial growth media-2 singlequot (ebm-2, cc-3156)/product/Cambrex
Average 90 stars, based on 1 article reviews
endothelial growth media-2 singlequot (ebm-2, cc-3156) - by Bioz Stars, 2026-03
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20μg/ml vegf antibody  (Millipore)
90
Millipore 20μg/ml vegf antibody
(A) Tubule formation assays were performed with HUVEC <t>and</t> <t>KSHV-HUVEC</t> in endothelial cell complete media, media without <t>VEGF,</t> or media without bFGF for four days. Images of the HUVEC and KSHV-HUVEC cells were taken under bright field microscopy. Additionally images of the KSHV-HUVEC (which express GFP) were taken under fluorescence microscopy. The following time-points were taken: 24 hours (panel A), 48 hours (panel B), 72 hours (panel C), and 96 hours (panel D) hours. Images shown were taken at 10X magnification. The tubules formed by KSHV-HUVEC appeared to be maintained on matrigel for a longer period of time compared with those formed by HUVEC. (B) Tubule formation assays were performed with HUVEC and KSHV-HUVEC in serum-free EBM-2 media, with or without anti-VEGF antibody. Images were taken after 16 hours at 4X magnification. Uninfected HUVEC could not form tubules in the presence of the blocking VEGF antibody. Although overall numbers of tubules was greatly reduced, KSHV-infected HUVEC could still form a few tubules in the presence of the blocking antibody.
20μg/Ml Vegf Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/20μg/ml vegf antibody/product/Millipore
Average 90 stars, based on 1 article reviews
20μg/ml vegf antibody - by Bioz Stars, 2026-03
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Image Search Results


hESC monolayer differentiation. hESC were differentiated in EGM2-MV for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).

Journal: Tissue Engineering. Part A

Article Title: Human Embryonic Stem Cell-Derived Mesoderm-like Epithelium Transitions to Mesenchymal Progenitor Cells

doi: 10.1089/ten.tea.2008.0351

Figure Lengend Snippet: hESC monolayer differentiation. hESC were differentiated in EGM2-MV for 20–30 days. (A) Within 3–5 days, hESC (arrowhead) began forming epithelial foci (arrow), (B) expanded in a circular pattern (arrow) until (C) the entire culture presented an epithelial phenotype. (D) The epithelial phenotype underwent EMT with passaging. (E) Time line for differentiation of hESC to epithelium and EMT. (10 × magnification, A–D).

Article Snippet: Differentiation procedure and culture When hESC cultured without feeders as described above reached ∼90% confluence, the 100 mm dishes were washed with PBS ++ (with Ca 2+ and Mg 2+ ) and replaced with 10 mL of fresh endothelial growth media 2 microvascular (EGM2-MV) (Lonza; 5% FBS, proprietary endothelial basal media 2 (EBM2) basal media and concentrations of bFGF, VEGF, EGF, and R 3 -IGF-1 25 ).

Techniques: Passaging

(A) Tubule formation assays were performed with HUVEC and KSHV-HUVEC in endothelial cell complete media, media without VEGF, or media without bFGF for four days. Images of the HUVEC and KSHV-HUVEC cells were taken under bright field microscopy. Additionally images of the KSHV-HUVEC (which express GFP) were taken under fluorescence microscopy. The following time-points were taken: 24 hours (panel A), 48 hours (panel B), 72 hours (panel C), and 96 hours (panel D) hours. Images shown were taken at 10X magnification. The tubules formed by KSHV-HUVEC appeared to be maintained on matrigel for a longer period of time compared with those formed by HUVEC. (B) Tubule formation assays were performed with HUVEC and KSHV-HUVEC in serum-free EBM-2 media, with or without anti-VEGF antibody. Images were taken after 16 hours at 4X magnification. Uninfected HUVEC could not form tubules in the presence of the blocking VEGF antibody. Although overall numbers of tubules was greatly reduced, KSHV-infected HUVEC could still form a few tubules in the presence of the blocking antibody.

Journal:

Article Title: KSHV confers a survival advantage to endothelial cells

doi: 10.1158/0008-5472.CAN-07-5988

Figure Lengend Snippet: (A) Tubule formation assays were performed with HUVEC and KSHV-HUVEC in endothelial cell complete media, media without VEGF, or media without bFGF for four days. Images of the HUVEC and KSHV-HUVEC cells were taken under bright field microscopy. Additionally images of the KSHV-HUVEC (which express GFP) were taken under fluorescence microscopy. The following time-points were taken: 24 hours (panel A), 48 hours (panel B), 72 hours (panel C), and 96 hours (panel D) hours. Images shown were taken at 10X magnification. The tubules formed by KSHV-HUVEC appeared to be maintained on matrigel for a longer period of time compared with those formed by HUVEC. (B) Tubule formation assays were performed with HUVEC and KSHV-HUVEC in serum-free EBM-2 media, with or without anti-VEGF antibody. Images were taken after 16 hours at 4X magnification. Uninfected HUVEC could not form tubules in the presence of the blocking VEGF antibody. Although overall numbers of tubules was greatly reduced, KSHV-infected HUVEC could still form a few tubules in the presence of the blocking antibody.

Article Snippet: For the VEGF antibody experiments, HUVEC and KSHV-HUVEC were resuspended in EBM-2 media or EBM-2 media containing 20μg/ml VEGF antibody (Sigma) ( 36 ).

Techniques: Microscopy, Fluorescence, Blocking Assay, Infection